Molecular Characterization of the<i>ZKT</i>Gene Encoding a Protein with PDZ, K-Box, and TPR Motifs in<i>Arabidopsis</i>
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- ISHIKAWA Atsushi
- Department of Bioscience, Fukui Prefectural University
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- TANAKA Hideaki
- Department of Bioscience, Fukui Prefectural University
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- KATO Chiyuki
- Department of Bioscience, Fukui Prefectural University
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- IWASAKI Yukimoto
- Department of Bioscience, Fukui Prefectural University
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- ASAHI Tadashi
- Department of Bioscience, Fukui Prefectural University
書誌事項
- タイトル別名
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- Molecular Characterization of the ZKT Gene Encoding a Protein with PDZ, K-Box, and TPR Motifs in Arabidopsis
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The ZKT gene from Arabidopsis encodes a polypeptide of 335 amino acid residues, with a calculated molecular mass of 37.4 kDa. ZKT is a member of a novel protein family present in the plant kingdom, which contains a PDZ, a K-box, and a TPR motif. A BLAST search indicated that the ZKT gene is a single gene in Arabidopsis and that ZKT homologs are present in soybean and rice but not in animals. The level of ZKT mRNA decreased after wounding. Antisera from rabbit immunized with recommbinant ZKT protein recognized a protein of 37 kDa in Arabidopsis. Western analysis with anti-ZKT antibody indicated that the level of ZKT protein does not change after wounding. The ZKT protein has consensus sequence motifs for phosphorylation. Immunoprecipitation with anti-ZKT antibody and western analysis with anti-phosphoamino acid antibody indicated that the ZKT protein is phosophorylated at the threonine and serine residues after wounding. These results suggest that the ZKT protein may act as a molecular adaptor regulated by phosphorylation in wound responses.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 69 (5), 972-978, 2005
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206474736768
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- NII論文ID
- 130000030329
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7322530
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- PubMed
- 15914918
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可