Stimulatory Factors for Enzymatic Biotin Synthesis from Dethiobiotin in Cell-free Extracts of Escherichia coli.

DOI Web Site PubMed 4 Citations 14 References

Bibliographic Information

Other Title
  • Stimulatory Factors for Enzymatic Biotin Synthesis from Dethiobiotin in Cell-freeExtracts of<i>Escherichia coli</i>

Search this article

Description

The activity of biotin synthesis from dethiobiotin was found in cell-free extracts of an Escherichia coli bio B transformant. Among the sulfur compounds tested, only S-adenosyl-L-methionine (Ado-Met) had a significant effect, while methionine and cysteine were inert. The activity was linearly stimulated by increasing protein concentration. When the dialyzed cell-free extracts were used for the reaction, NADP+, NADPH, and FAD among the well-known cofactors tested promoted the activity. Furthermore, in the presence of AdoMet, cysteine was apparently effective for biotin synthetic activity.

Journal

Citations (4)*help

See more

References(14)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top