Prevention of Intestinal Infection by Glycomacropeptide
-
- NAKAJIMA Kouhei
- Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
-
- TAMURA Norimitsu
- Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
-
- KOBAYASHI-HATTORI Kazuo
- Division of Microbiology, The National Institute of Health Sciences
-
- YOSHIDA Tadashi
- Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
-
- HARA-KUDO Yukiko
- Division of Microbiology, The National Institute of Health Sciences
-
- IKEDO Masanari
- Eiken Chemical Co., Ltd.
-
- SUGITA-KONISHI Yoshiko
- Division of Microbiology, The National Institute of Health Sciences
-
- HATTORI Makoto
- Institute of Symbiotic Science and Technology, Division of Agriscience and Bioscience, Tokyo University of Agriculture and Technology
Search this article
Description
The preventive effects of glycomacropeptide (GMP) against intestinal infection were investigated, and conjugates of GMP with xylooligosaccharide (XOS) and carboxymethyldextran (CMD) were prepared by the Maillard reaction to enhance the effect of GMP. The binding ability of GMP to intestinal pathogenic bacteria was evaluated by a binding assay with biotinylated bacteria. GMP showed the ability to bind to Salmonella enteritidis and enterohemorrhagic Escherichia coli O157:H7 (EHEC O157). This binding ability was decreased by a sialidase treatment and completely eliminated by periodate oxidation. These results indicate that such carbohydrate moieties as sialic acid in GMP are involved in binding to S. enteritidis and EHEC O157. The preventive effect of GMP on the adhesion of pathogenic bacteria to Caco-2 cells was also investigated. GMP showed an inhibitory effect on the adhesion of EHEC O157 in a dose-dependent manner, although it was not a potent inhibitor of the adhesion of Salmonella infection. However, in the case of Salmonella infection, GMP–XOS and GMP–CMD significantly suppressed IL-8 production which was the index of infection. Our results indicate GMP to be a promising agent for preventing intestinal infection.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 69 (12), 2294-2301, 2005
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001206475012608
-
- NII Article ID
- 130000030443
-
- NII Book ID
- AA10824164
-
- COI
- 1:CAS:528:DC%2BD28XjtVarsQ%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 7755867
-
- PubMed
- 16377886
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed