Nucleotide Sequence of the Gene Encoding the Precursor Protein of Pepstatin insensitive Acid Protease B, Scytalidopepsin B, from Scytalidium lignicolum.

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  • ODA Naoko
    Department of Applied Microbial Technology, Kumamoto Institute of Technology
  • GOTOH Yoshikazu
    Department of Applied Microbial Technology, Kumamoto Institute of Technology
  • OYAMA Hiroshi
    Department of Applied Microbial Technology, Kumamoto Institute of Technology
  • MURAO Sawao
    Department of Applied Microbial Technology, Kumamoto Institute of Technology
  • ODA Kohei
    Faculty of Textile Science, Kyoto Institute of Technology
  • TSURU Daisuke
    Department of Applied Microbial Technology, Kumamoto Institute of Technology

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  • Nucleotide Sequence of the Gene Encodin
  • Nucleotide Sequence of the Gene Encoding the Precursor Protein of Pepstatin insensitive Acid Protease B, Scytalidopepsin B, from<i>Scytalidium lignicolum</i>

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  A chromosomal DNA of Scytalidium lignicolum was digested with Sau3AI. The digest was self-ligated and amplified by inverse PCR procedure using primers designed based on the nucleotide sequences of up- and down-stream regions of an intron present in the scytalidopepsin B gene. Analysis of the nucleotide sequence of PCR product (700 bp) showed that the enzyme is synthesized as a precursor protein consisting of the prepro- and mature enzyme regions. The deduced amino acid sequence was highly similar to those of aspergillopepsin A and recently reported endothiapepsins B and C, but quite different from those of pepstatin-insensitive bacterial acid proteases and the pepstatin-sensitive aspartic protease family.<br>

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