Atomic Force Microscopic Observation of Escherichia coli Ribosomes in Solution

DOI Web Site Web Site 2 Citations 29 References
  • MATSUURA Toshihiko
    Institute of Scientific and Industrial Research, Osaka University Technology Research Laboratory, Shimadzu Corporation
  • TANAKA Hiroyuki
    Institute of Scientific and Industrial Research, Osaka University
  • MATSUMOTO Takuya
    Institute of Scientific and Industrial Research, Osaka University
  • KAWAI Tomoji
    Institute of Scientific and Industrial Research, Osaka University

Bibliographic Information

Other Title
  • Atomic Force Microscopic Observation of<i>Escherichia coli</i>Ribosomes in Solution

Search this article

Abstract

Ribosomes of Escherichia coli were visualized in buffer solution by atomic force microscopy (AFM). A series of time-dependent AFM images showed that ribosomes spontaneously adsorb on mica. Although ribosomes observed in air are forced to flatten on the surface, the height of ribosomal particles obtained under a physiological buffer solution is 21.8±0.5 nm, which is consistent with the ideal diameter. We succeeded in observing single ribosomes in a near-native condition.

Journal

Citations (2)*help

See more

References(29)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top