Improvement of Desulfurization Activity in Rhodococcus erythropolis KA2-5-1 by Genetic Engineering

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  • HIRASAWA Kazuaki
    Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center
  • ISHII Yoshitaka
    Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center
  • KOBAYASHI Morio
    Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center
  • KOIZUMI Kenichi
    Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center
  • MARUHASHI Kenji
    Bio-Refining Process Laboratory, Advanced Technology and Research Institute, Petroleum Energy Center

書誌事項

タイトル別名
  • Improvement of Desulfurization Acitivity in Rhodococcus erythropolis KA2-5-1 by Genetic Engineering.
  • Improvememt of Desulfurization Activity in Rhodococcus erythropolis KA2-5-1 by Genetic Engineering

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Rhodococus erythropolis KA2-5-1 can desulfurize dibenzothiophene (DBT) into 2-hydroxybiphenyl. A cryptic plasmid, pRC4, which was derived from R. rhodochrous IFO3338, was combined with an Escherichia coli vector to construct an E. coli-Rhodococcus shuttle vector. The complete nucleotide sequence of 2582-bp pRC4 was analyzed. Based on the characteristics of its putative replication genes, pRC4 was assigned to the family of pAL5000-related replicons. The desulfurization gene cluster, dszABC, and the related reductase gene, dszD, cloned from KA2-5-1, were reintroduced into KA2-5-1 and efficiently expressed. The DBT desulfurization ability of the transformant carrying two dszABC clusters and one dszD on the vector was about 4-fold higher than that of the parent strain, and the transformant also showed improved desulfurization activity for light gas oil (LGO). Sulfur components in LGO before and after the reaction were analyzed with gas chromatography-atomic emission detection.

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