Molecular Cloning and Ethylene-Inducible Expression of Chib1 Chitinase from Soybean (Glycine max (L.) Merr.).

DOI Web Site Web Site PubMed 2 Citations 33 References
  • WATANABE Akira
    Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba
  • HAI NONG Van
    Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba Institute of Biotechnology, National Center for Natural Science and Technology
  • ZHANG Deyu
    Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba
  • ARAHIRA Masaomi
    Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba
  • ASARE YEBOAH Nana
    Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba
  • UDAKA Kyoko
    Tokyo Kasei University
  • FUKAZAWA Chikafusa
    Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba

Bibliographic Information

Other Title
  • Molecular Cloning and Ethylene-Inducible Expression of<i>Chi</i>b1 Chitinase from Soybean (<i>Glycine max</i>(L.) Merr.)

Search this article

Description

  A soybean seed-specific PR-8 chitinase, named Chib2, has a markedly extended C-terminal segment compared to other plant Chib1 homologues of the PR-8 chitinase family known to date.<br>   To further characterize the molecular structure and the expression pattern of this chitinase family, we cloned two typical Chib1-similar cDNAs (Chib1-1 and Chib1-2) from soybeans by PCR-cloning techniques. The deduced primary sequence of Chib1-1 chitinase is composed of a signal peptide segment (26 amino acid residues) and a mature 273 amino acid sequence (calculated molecular mass 28,794, calculated pI 3.7). This Chib1-1 enzyme is more than 90% identical to Chib1-2 chitinase but is below 50% identical to Chib2 enzyme. Thus, we confirmed the occurrence of two distinct classes, Chib1 and Chib2 in the plant PR-8 chitinase family. The Chib1 genes, interrupted by one intron, were found to be up-regulated in response to ethylene in stems and leaves, but scarcely expressed in developing soybean seeds. Chib1 chitinases may be responsible for protecting the plant body from various pathogenic attacks.<br>

Journal

Citations (2)*help

See more

References(33)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top