Molecular Cloning and Characterization of the Fructooligosaccharide-Producing β-Fructofuranosidase Gene from Aspergillus niger ATCC 20611
-
- YANAI Koji
- Bio Science Laboratories, Meiji Seika Kaisha, Ltd. Present address: Pharmaceutical Technology Laboratories, Meiji Seika Kaisha, Ltd.
-
- NAKANE Akitaka
- Bio Science Laboratories, Meiji Seika Kaisha, Ltd.
-
- KAWATE Akemi
- Bio Science Laboratories, Meiji Seika Kaisha, Ltd.
-
- HIRAYAMA Masao
- Bio Science Laboratories, Meiji Seika Kaisha, Ltd.
書誌事項
- タイトル別名
-
- Molecular Cloning and Characterization of the Fructooligosaccharide-Producing .BETA.-Fructofuranosidase Gene from Aspergillus niger ATCC 20611.
- Molecular Cloning and Characterization of the Fructooligosaccharide Producing ベータ Fructofuranosidase Gene from Aspergillus niger ATCC 20611
この論文をさがす
抄録
The fopA gene encoding a fructooligosaccharide-producing β-fructofuranosidase was isolated from Aspergillus niger ATCC 20611. The primary structure deduced from the nucleotide sequence showed considerable similarity to those of two other β-fructofuranosidases from A. niger, but the fopA gene product had several amino acid insertions and an extra C-terminal polypeptide consisting of 38 amino acids that could not be found in the two others. We could successfully express the fopA gene in S. cerevisiae and the fopA gene product obtained from the culture supernatant of the S. cerevisiae transformant had similar characteristics to the β-fructofuranosidase purified from A. niger ATCC 20611. However, we could not detect any β-fructofuranosidase activity in either the culture supernatant or cell lysate when the C-terminal truncated fopA gene product by 38 amino acids was used to transform S. cerevisiae. In western analysis of those samples, there was no protein product that is cross-reacted with anti-β-fructofuranosidase antibody. These results suggested that the C-terminal region of the fopA gene product consisting of 38 amino acids was essential for the enzyme production.
収録刊行物
-
- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
-
Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 65 (4), 766-773, 2001
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206475865600
-
- NII論文ID
- 110002680343
-
- NII書誌ID
- AA10824164
-
- COI
- 1:CAS:528:DC%2BD3MXjsVags7Y%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 5768779
-
- PubMed
- 11388451
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可