Streptokinase Recovery by Cross-Flow Microfiltration: Study of Enzyme Denaturation.
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- タイトル別名
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- Streptokinase Recovery by Cross-Flow Mi
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During streptokinase (SK) recovery by cross-flow microfiltration (CFMF), a loss of activity of 14.4% was observed, after the initial volume was concentrated 8-fold (VCF=8.0); 51.5% of the activity was recovered in the filtrate and 34.1% remained in the retentate. Immunological experiments using polyclonal antibodies against SK have demonstrated that SK activity loss during CFMF processes could be related to denaturation of SK, forming molecules of lower or no activity. Accumulation of denatured SK in the retentate suggests that denaturation could be the results of an aggregation phenomenon (as has been demonstrated by light scattering and chromatographic studies), leading to the formation of protein aggregates that are retained by the microfiltration (MF) membrane, affecting the fouling phenomenon and the concentration in the retentate of permeable molecules.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (8), 1240-1243, 1997
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206475963776
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- NII論文ID
- 110002678689
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4287833
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- PubMed
- 9301102
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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- 使用不可