Effects of Rooibos Tea Extract on Antigen-specific Antibody Production and Cytokine Generation in Vitro and in Vivo.
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- KUNISHIRO Kazuyoshi
- Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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- TAI Akihiro
- Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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- YAMAMOTO Itaru
- Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University
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抄録
Rooibos tea contains a large amount of flavonoids and acts as a potent antioxidant. In this study, we examined the effects of Rooibos tea extract on antigenspecific antibody production and cytokine generation in vitro and in vivo. The primary in vitro anti-ovalbumin (anti-OVA) or sheep red blood cell (SRBC) antibody production in murine splenocytes was markedly stimulated by the addition of the tea extract at concentrations of 1-100 μg/ml. On the other hand, a nonspecific antibody response elicited with lipopolysaccharide (LPS) in purified splenic B-cells was not modified by the extract. Rooibos tea extract caused an increase in the generation of interleukin 2 (IL-2) both in OVA- and anti-CD3-primed splenocytes at concentrations ranging from 10 μg/ml to 1000 μg/ml. In contrast, this tea extract suppressed the generation of interleukin 4 (IL-4) in OVA-primed splenocytes. Moreover, the reduction of OVA-induced antibody production in serum of the cyclosporin A (CyA)-treated rats can be significantly restored and the IL-2 generation in murine splenocytes was stimulated, following oral administrations of Rooibos tea extract. Thus, our findings suggested that Rooibos tea extract may facilitate the antigen-specific antibody production through selective augmentation of IL-2 generation both in vitro and in vivo. Collectively, Rooibos tea intake may be of value in prophylaxis of the diseases involving a severe defect in Th1 immune response such as cancer, allergy, AIDS, and other infections.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 65 (10), 2137-2145, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206475972096
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- NII論文ID
- 110002693252
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3MXotFantbk%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5968996
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- PubMed
- 11758901
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可