Occurrence of Lewis a Epitope in N-Glycans of a Glycoallergen, Jun a 1, from Mountain Cedar (Juniperus ashei) Pollen
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- KIMURA Yoshinobu
- Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University
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- KAMAMOTO Maiko
- Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
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- MAEDA Megumi
- Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University
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- OKANO Mitsuhiro
- Department of Otolaryngology-Head and Neck Surgery, Okayama University Graduate School of Medicine and Dentistry
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- YOKOYAMA Minehiko
- Meiji Co.
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- KINO Kosuke
- Meiji Co.
Bibliographic Information
- Other Title
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- Occurrence of Lewis a Epitope in<i>N</i>-Glycans of a Glycoallergen, Jun a 1, from Mountain Cedar (<i>Juniperus ashei</i>) Pollen
- Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar pollen
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Abstract
We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1–3(Fucα1–4)GlcNAcβ1–) occurs in the N-glycans of the pollen allergens.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 69 (1), 137-144, 2005
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001206476264576
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- NII Article ID
- 130000030564
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7237392
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- PubMed
- 15665478
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed