Efficient Release of Overproduced Gene Products from<i>Escherichia coli</i>BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages

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  • IIDA Yuichiro
    <i>Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University</i>
  • MATSUDA Yoshinori
    <i>Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University</i>
  • SAITO Ryuichiro
    <i>Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University</i>
  • NAKASATO Masanori
    <i>Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University</i>
  • NONOMURA Teruo
    <i>Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University</i>
  • KAKUTANI Koji
    <i>Pharmaceutical Research and Technology Institute, Kinki University</i>
  • TOSA Yukio
    <i>Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University</i>
  • MAYAMA Shigeyuki
    <i>Laboratory of Plant Pathology, Faculty of Agriculture, Kobe University</i>
  • TOYODA Hideyoshi
    <i>Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kinki University</i>

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  • Efficient Release of Overproduced Gene Products from Escherichia coli BL21(DE3) by Lytic Infection with Newly Isolated Bacteriophages.

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  Overproduced proteins from Escherichia coli BL21(DE3) were efficiently released with virulent bacteriophages. Leviviridae-like bacteriophages were isolated from soil and used to lyse BL21(DE3) cells transformed with β-glucosidase, chitinase, or chitosanase genes. This method caused lysis of bacterial cells similar to that by conventional sonication and enabled us to effectively recover and purify the enzymes.<br>

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