Cell-lytic Activity of Tobacco BY-2 Induced by a Fungal Elicitor from Alternaria alternata Attributed to the Expression of a Class 1 β-1,3-Glucanase Gene

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  • SHINYA Tomonori
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • GONDO Shinobu
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • IIJIMA Hiroshi
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • HANAI Kazunari
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • MATSUOKA Hideaki
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
  • SAITO Mikako
    Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology

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タイトル別名
  • Cell-lytic Activity of Tobacco BY-2 Induced by a Fungal Elicitor from Alternaria alternata Attributed to the Expression of a Class I .BETA.-1,3-Glucanase Gene
  • Cell lytic Activity of Tobacco BY 2 Induced by a Fungal Elicitor from Alternaria alternata Attributed to the Expression of a Class 1 ベータ 1 3 Glucanase Gene
  • Cell-lytic Activity of Tobacco BY-2 Induced by a Fungal Elicitor from<i>Alternaria alternata</i>Attributed to the Expression of a Class I β-1,3-Glucanase Gene

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Stress-induced cell-lytic activity was found in tobacco BY-2 cells treated with various stresses. Among 14 stresses, an elicitor fraction isolated from Alternaria alternata showed the highest inducing activity. Cell-lytic activity increased for 72 h even in the control sample, treated with distilled water, and several isozymes of β-1,3-glucanases and chitinases were found to be involved in it. In contrast, cell-lytic activity in BY-2 cells treated with a fungal elicitor reached a higher level after 60 h. The principal enzymes specifically involved in this stress-induced portion are speculated to be basic β-1,3-glucanases. A class I β-1,3-glucanase gene (glu1) was found to be the specific gene for the stress-induced cell-lytic activity. Its expression became observable at 24 h, and the intensity reached a maximum at about 60–72 h. The glu1 was thus assigned as a late gene. Its role in the stress response is discussed in conjunction with earlier genes such as chitinases.

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