Microbial Synthesis of trans Isomer of Eicosapentaenoic Acid(EPA) from the Chemically Synthesized Trans Isomer of Linolenic Acid by a Δ12 Desaturase-defective Mutant of Mortierella alpina 1S-4
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- SHIRASAKA Norifumi
- <i>Department of Food and Nutrition, Faculty of Agriculture, Kinki University</i>
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- MIYAMOTO Shigenori
- <i>Department of Food and Nutrition, Faculty of Agriculture, Kinki University</i>
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- MURAKAMI Tetsuo
- <i>Department of Food and Nutrition, Faculty of Agriculture, Kinki University</i>
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- YOSHIZUMI Hajime
- <i>Department of Food and Nutrition, Faculty of Agriculture, Kinki University</i>
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- SHIMIZU Sakayu
- <i>Division of Applied Life Science, Graduate School of Agriculture, Kyoto University</i>
書誌事項
- タイトル別名
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- Microbial Synthesis of trans Isomer of Eicosapentaenoic Acid (EPA) from the Chemically Synthesized Trans Isomer of Linolenic Acid by a .DELTA.12 Desaturase-defective Mutant of Mortierella alpina 1S-4
- Microbial Synthesis of trans Isomer of Eicosapentaenoic Acid EPA from the Chemically Synthesized Trans Isomer of Linolenic Acid by a デルタ 12 Desaturase defective Mutant of Mortierella alpina 1S 4
- Microbial Synthesis of<i>trans</i>Isomer of Eicosapentaenoic Acid (EPA) from the Chemically Synthesized Trans Isomer of Linolenic Acid by a<i>Δ</i>12 Desaturase-defective Mutant of…
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説明
The mono trans geometrical isomer of eicosapentaenoic acid, 5c,8c,11c,14c,17t-eicosapenntaenoic acid (20:5Δ5c,8c,11c,14c,17t), was synthesized by fatty acid microbial conversion using a Δ12-desaturase defective mutant of an arachidonic acid (AA)-producing fungus, Mortierella alpina 1S-4. The substrate for the bioconversion, a geometrical isomer of linolenic acid, was prepared by isomerization of linseed oil methyl ester by the nitrous acid method, followed by purification on a AgNO3-silica gel column. The structure and double bond geometry were identified after hydrazine reduction followed by permanganate oxidation to 20:5Δ5c, 8c,11c,14c,17t. The biosynthetic route from 18:3Δ6c, 9c,12t to 20:5Δ5c,8c,11c,14c,17t was presumed to mimic the route from linoleic acid to arachidonic acid.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 67 (5), 1164-1167, 2003
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206476400256
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- NII論文ID
- 110002694198
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3sXktlygu7s%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 6546089
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- PubMed
- 12834302
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- 本文言語コード
- en
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- 資料種別
- journal article
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