Microbial Synthesis of trans Isomer of Eicosapentaenoic Acid(EPA) from the Chemically Synthesized Trans Isomer of Linolenic Acid by a Δ12 Desaturase-defective Mutant of Mortierella alpina 1S-4

  • SHIRASAKA Norifumi
    <i>Department of Food and Nutrition, Faculty of Agriculture, Kinki University</i>
  • MIYAMOTO Shigenori
    <i>Department of Food and Nutrition, Faculty of Agriculture, Kinki University</i>
  • MURAKAMI Tetsuo
    <i>Department of Food and Nutrition, Faculty of Agriculture, Kinki University</i>
  • YOSHIZUMI Hajime
    <i>Department of Food and Nutrition, Faculty of Agriculture, Kinki University</i>
  • SHIMIZU Sakayu
    <i>Division of Applied Life Science, Graduate School of Agriculture, Kyoto University</i>

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タイトル別名
  • Microbial Synthesis of trans Isomer of Eicosapentaenoic Acid (EPA) from the Chemically Synthesized Trans Isomer of Linolenic Acid by a .DELTA.12 Desaturase-defective Mutant of Mortierella alpina 1S-4
  • Microbial Synthesis of trans Isomer of Eicosapentaenoic Acid EPA from the Chemically Synthesized Trans Isomer of Linolenic Acid by a デルタ 12 Desaturase defective Mutant of Mortierella alpina 1S 4
  • Microbial Synthesis of<i>trans</i>Isomer of Eicosapentaenoic Acid (EPA) from the Chemically Synthesized Trans Isomer of Linolenic Acid by a<i>Δ</i>12 Desaturase-defective Mutant of…

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説明

The mono trans geometrical isomer of eicosapentaenoic acid, 5c,8c,11c,14c,17t-eicosapenntaenoic acid (20:5Δ5c,8c,11c,14c,17t), was synthesized by fatty acid microbial conversion using a Δ12-desaturase defective mutant of an arachidonic acid (AA)-producing fungus, Mortierella alpina 1S-4. The substrate for the bioconversion, a geometrical isomer of linolenic acid, was prepared by isomerization of linseed oil methyl ester by the nitrous acid method, followed by purification on a AgNO3-silica gel column. The structure and double bond geometry were identified after hydrazine reduction followed by permanganate oxidation to 20:5Δ5c, 8c,11c,14c,17t. The biosynthetic route from 18:3Δ6c, 9c,12t to 20:5Δ5c,8c,11c,14c,17t was presumed to mimic the route from linoleic acid to arachidonic acid.<br>

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