Improved Production of Enzymes, Which Are Expressed under the Pho Regulon Promoter, in the<i>rmf</i>Gene (encoding ribosome modulation factor) Disruptant of<i>Escherichia coli</i>

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  • IMAIZUMI Akira
    Laboratories of Fermentation and Biotechnology, AJINOMOTO Co., Inc.
  • KOSEKI Chie
    Laboratories of Fermentation and Biotechnology, AJINOMOTO Co., Inc.
  • MATSUI Kazuhiko
    Laboratories of Fermentation and Biotechnology, AJINOMOTO Co., Inc.
  • KOJIMA Hiroyuki
    Laboratories of Fermentation and Biotechnology, AJINOMOTO Co., Inc.

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  • Improved Production of Enzymes, Which Are Expressed under the Pho Regulon Promoter, in the rmf Gene (encoding ribosome modulation factor) Disruptant of Escherichia coli

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Using a DNA macroarray, we investigated the effects of rmf gene (encoding ribosome modulation factor) disruption on gene expression profiles in Escherichia coli. This strain showed a phosphate-starvation-like response in gene expression even under phosphate sufficient conditions; significant upregulation of the Pho regulon genes was observed. Further, the production of alkaline phosphatase, a product of the Pho regulon gene, phoA, increased in the rmf disruptant under a Pi sufficient condition. Furthermore, production of PhoC acid phosphatase/nucleoside pyrophosphate phosphotransferase derived from Morganella morganii also increased significantly in the rmf disruptant. We concluded that host modification by the rmf gene disruption has potential benefit in industrial enzyme production using Escherichia coli.

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