Construction and Characterization of a Deletion Mutant of<i>gpd2</i>That Encodes an Isozyme of NADH-Dependent Glycerol-3-phosphate Dehydrogenase in Fission Yeast
書誌事項
- タイトル別名
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- Construction and Characterization of a Deletion Mutant of gpd2 That Encodes an Isozyme of NADH-Dependent Glycerol-3-phosphate Dehydrogenase in Fission Yeast.
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説明
Schizosaccharomyces pombe has two genes each encoding an isozyme of NADH-dependent glycerol-3-phosphate dehydrogenases (gpd1+ and gpd2+). To gain an insight into the function of these genes, here we constructed a gpd2 deletion mutant, in addition to the previously constructed gpd1 deletion mutant. We showed that the gpd1+ and gpd2+ gene-products are both funcional in terms of the de novo glycerol synthesis. Furthermore, the gpd1+-mediated glycerol production is primarily responsible for the osmoregulation, but the gpd2+ gene is not. Interestingly, however, the gpd2 deletion mutant had histidine- or lysine-auxotrophy for growth on a minimal medium.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 60 (5), 918-920, 1996
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206476618752
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- NII論文ID
- 110002678159
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK28Xjtl2qsb8%3D
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- ISSN
- 13476947
- 09168451
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- PubMed
- 8704325
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- JaLC
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- PubMed
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- 使用不可