Inhibition of E-Cadherin Dependent Cell-Cell Contact Promotes MUC5AC Mucin Production through the Activation of Epidermal Growth Factor Receptors
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- IWASHITA Jun
- Molecular Biology Laboratory, Faculty of Bioresource Sciences, Akita Prefectural University
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- OSE Kyohei
- Molecular Biology Laboratory, Faculty of Bioresource Sciences, Akita Prefectural University
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- ITO Hiroki
- Molecular Biology Laboratory, Faculty of Bioresource Sciences, Akita Prefectural University
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- MURATA Jun
- Molecular Biology Laboratory, Faculty of Bioresource Sciences, Akita Prefectural University
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- ABE Tatsuya
- Molecular Biology Laboratory, Faculty of Bioresource Sciences, Akita Prefectural University
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Mucin production by epithelial cells is modulated by many soluble factors, including epidermal growth factor (EGF). E-Cadherin promotes EGF receptor (EGFR)-mediated MUC5AC mucin production in airway epithelial cells in dense cultures, suggesting the involvement of E-cadherin in activating EGFRs and mucin production. However, the role of E-cadherin in modulating mucin production is not completely understood. We examined its role in MUC5AC production in a human lung epithelial cell line, NCI-H292. Treatment of low density NCI-H292 cells with an anti-E-cadherin monoclonal antibody (SHE78-7) inhibited cell-cell contact in the dispersed colonies, but promoted MUC5AC production. Furthermore, treatment of the NCI-H292 cells with anti-E-cadherin antibody stimulated phosphorylation of extracellular signal-regulated kinase (ERK). The enhanced production of MUC5AC was inhibited with an EGFR inhibitor and with a MEK inhibitor, but not with a Src family kinase inhibitor. These results suggest that inhibition of E-cadherin activates EGFRs independently of Src and promotes MUC5AC production through the ERK signaling pathway in sparsely cultured NCI-H292 cells.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (4), 688-693, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206476916352
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- NII論文ID
- 10028271470
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11070966
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可