Genomic Recombination through Plasmid-Encoded Recombinase Enhances Hemolytic Activity and Adherence to Epithelial Cells in the Periodontopathogenic Bacterium<i>Eikenella corrodens</i>
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- MATSUNAGA Tetsuro
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- NAKAYUKI Ayumi
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- SAITO Yuki
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- KATO Akio
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
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- NOIRI Yuichiro
- Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
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- EBISU Shigeyuki
- Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry
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- AZAKAMI Hiroyuki
- Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
書誌事項
- タイトル別名
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- Genomic Recombination through Plasmid-Encoded Recombinase Enhances Hemolytic Activity and Adherence to Epithelial Cells in the Periodontopathogenic Bacterium Eikenella corrodens
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抄録
The periodontopathogenic bacterium Eikenella corrodens has an N-acetyl-D-galactosamine (GalNAc)-specific lectin, that contributes significantly to the pathogenicity of the bacterium. Recently, we reported that plasmid-mediated genomic recombination enhances the activity of this lectin. In this study, we investigated the effects of genomic recombination on certain virulence factors. Introduction of the recombinase gene resulted in hemolysis and significantly increased bacterial adhesion to epithelial cells. It was suggested that the enhanced adhesion was attributable to increased lectin activity due to genomic recombination, because it was inhibited by the addition of GalNAc. In contrast, invasion of the epithelial cells was remarkably reduced by genomic recombination. Although we assumed that this decrease in invasion resulted from a loss of type-IV pili, the phase variant did not show any decrease in invasion activity. This suggests that type-IV pili do not contribute to the invasive ability of E. corrodens. Our results suggest that genomic recombination enhances the pathogenicity of E. corrodens.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (4), 748-751, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206476935936
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- NII論文ID
- 10028271753
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11071077
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可