A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans
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- INOUE Takahiro
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- YOSHIDA Takashi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- WADA Kei
- Department of Biological Sciences, Graduate School of Science, Osaka University
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- DAIFUKU Takashi
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- FUKUYAMA Keiichi
- Department of Biological Sciences, Graduate School of Science, Osaka University
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- SAKO Yoshihiko
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
Bibliographic Information
- Other Title
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- A simple, large-scale overexpression method of deriving carbon monoxide dehydrogenase 2 from thermophilic bacterium Carboxydothermus hydrogenoformans
- A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium<i>Carboxydothermus hydrogenoformans</i>
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Abstract
We established an Na2S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO2 reduction activity, of 16.9 U/mg.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 75 (7), 1392-1394, 2011
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206476986112
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- NII Article ID
- 10029329599
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 11175267
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- PubMed
- 21737917
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed