A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium Carboxydothermus hydrogenoformans

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  • INOUE Takahiro
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
  • YOSHIDA Takashi
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
  • WADA Kei
    Department of Biological Sciences, Graduate School of Science, Osaka University
  • DAIFUKU Takashi
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
  • FUKUYAMA Keiichi
    Department of Biological Sciences, Graduate School of Science, Osaka University
  • SAKO Yoshihiko
    Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

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  • A simple, large-scale overexpression method of deriving carbon monoxide dehydrogenase 2 from thermophilic bacterium Carboxydothermus hydrogenoformans
  • A Simple, Large-Scale Overexpression Method of Deriving Carbon Monoxide Dehydrogenase II from Thermophilic Bacterium<i>Carboxydothermus hydrogenoformans</i>

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Abstract

We established an Na2S-free, large-scale overexpression system of deriving CODH II from thermophilic bacterium Carboxydothermus hydrogenoformans in Escherichia coli using a large-scale fermentor. Recombinant-CODH II showed a CO oxidation activity of 9,600 U/mg. In addition, recombinant-CODH II exhibited considerable CO2 reduction activity, of 16.9 U/mg.

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