Novel Fluorescent Probe for Analysis of Hydroperoxides Based on Boron Dipyrromethane Fluorophore

  • INOUE Naoko
    Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Katayanagi Institute, Tokyo University of Technology
  • SUZUKI Yoshio
    Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Katayanagi Institute, Tokyo University of Technology
  • YOKOYAMA Kenji
    Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Katayanagi Institute, Tokyo University of Technology Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST)
  • KARUBE Isao
    Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology (AIST), Katayanagi Institute, Tokyo University of Technology

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説明

A new reagent, N-[2-(diphenylphosphino)ethyl]-4-(1,3,5,7-tetramethyl-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-8-yl)benzamide (DPPEA-BODIPY), was designed and synthesized for analysis of hydroperoxides. DPPEA-BODIPY fluoresces at low levels in the visible range (λex⁄λem = 502 nm/515 nm) and reacts with hydroperoxides to produce DPPEA-BODIPY oxide, which fluoresces at high levels. The fluorescence intensity of the reaction mixture was observed to be linearly related to the methyl linoleate hydroperoxide (MeLOOH) concentration.

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