<I>Ameyamaea chiangmaiensis</I> gen. nov., sp. nov., an Acetic Acid Bacterium in the α<I>-Proteobacteria</I>

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  • YUKPHAN Pattaraporn
    BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)
  • MALIMAS Taweesak
    BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)
  • MURAMATSU Yuki
    Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE)
  • TAKAHASHI Mai
    Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE)
  • KANEYASU Mika
    Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE)
  • POTACHAROEN Wanchern
    BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)
  • TANASUPAWAT Somboon
    Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University
  • NAKAGAWA Yasuyoshi
    Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE)
  • HAMANA Koei
    School of Health Sciences, Faculty of Medicine, Gunma University
  • TAHARA Yasutaka
    Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University
  • SUZUKI Ken-ichiro
    Biological Resource Center (NBRC), Department of Biotechnology, National Institute of Technology and Evaluation (NITE)
  • TANTICHAROEN Morakot
    BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)
  • YAMADA Yuzo
    BIOTEC Culture Collection (BCC), National Center for Genetic Engineering and Biotechnology (BIOTEC)

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  • Ameyamaea chiangmaiensis gen. nov., sp. nov., an acetic acid bacterium in the α-Proteobacteria
  • Ameyamaea chiangmaiensis gen nov sp nov an acetic acid bacterium in the a Proteobacteria

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Abstract

Two isolates, AC04T and AC05, were isolated from the flowers of red ginger collected in Chiang Mai, Thailand. In phylogenetic trees based on 16S rRNA gene sequences, the two isolates were included within a lineage comprised of the genera Acidomonas, Gluconacetobacter, Asaia, Kozakia, Swaminathania, Neoasaia, Granulibacter, and Tanticharoenia, and they formed an independent cluster along with the type strain of Tanticharoenia sakaeratensis. The calculated pair-wise sequence similarities of isolate AC04T were 97.8–92.5% to the type strains of the type species of the 11 genera of acetic acid bacteria. The DNA base composition was 66.0–66.1 mol % G+C with a range of 0.1 mol %. A single-stranded, labeled DNA from isolate AC04T presented levels of DNA-DNA hybridization of 100, 85, 4, and 3% respectively to DNAs from isolates AC04T and AC05 and the type strains of Tanticharoenia sakaeratensis and Gluconacetobacter liquefaciens. The two isolates were unique morphologically in polar flagellation and physiologically in intense acetate oxidation to carbon dioxide and water and weak lactate oxidation. The intensity in acetate oxidation almost equaled that of the type strain of Acetobacter aceti. The two isolates had Q-10. Isolate AC04T was discriminated from the type strains of the type species of the 11 genera by 16S rRNA gene restriction analysis using restriction endonucleases TaqI and Hin6I. The unique phylogenetic, genetic, morphological, physiological, and biochemical characteristics obtained indicate that the two isolates can be classified into a separate genus, and Ameyamaea chiangmaiensis gen. nov., sp. nov. is proposed. The type strain is isolate AC04T (=BCC 15744T, =NBRC 103196T), which has a DNA G+C content of 66.0 mol %.

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