Changes in Nitric Oxide Production Levels and Expression of Nitric Oxide Synthase Isoforms in the Rat Uterus during Pregnancy
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- SUZUKI Takehito
- Graduate School of Veterinary Medicine, Azabu University
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- MORI Chiemi
- Graduate School of Veterinary Medicine, Azabu University
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- YOSHIKAWA Hiroshi
- Graduate School of Veterinary Medicine, Azabu University
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- MIYAZAKI Yoko
- Graduate School of Veterinary Medicine, Azabu University
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- KANSAKU Norio
- Graduate School of Veterinary Medicine, Azabu University
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- TANAKA Kazuaki
- Graduate School of Veterinary Medicine, Azabu University Research Institute of Biosciences, Azabu University
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- MORITA Hidetoshi
- Graduate School of Veterinary Medicine, Azabu University
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- TAKIZAWA Tatsuya
- Graduate School of Veterinary Medicine, Azabu University Research Institute of Biosciences, Azabu University
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Abstract
We clarified nitric oxide (NO) production in the rat uterus by electron paramagnetic resonance spectroscopy and with Fe-N-(dithiocarboxy) sarcosine complex (an NO-trapping reagent). We examined changes in NO production in the whole uterus, decidua, and myometrium (gestational days 13.5–21.5). The expression of nitric oxide synthase (NOS) isoforms was also examined by quantitative reverse transcription-polymerase chain reaction. The uterine NO levels were low on day 13.5, peaked on day 17.5, and thereafter decreased significantly. The NO production levels in the decidua and myometrium were the same on day 13.5, but the levels in the decidua were 2- to 4-fold higher than those in the myometrium from day 15.5 onwards. The NOS-2 mRNA expression pattern correlated well with changes in the NO levels in the decidua, whereas the NOS-3 mRNA was expressed constantly during gestation. Thus NOS-2-generated NO in the decidua contributed significantly to uterine NO levels.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 73 (10), 2163-2166, 2009
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206477034880
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- NII Article ID
- 10027546391
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 10418949
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed