Transplantation of a Mammary Stromal Cell Line into a Mammary Fat Pad: Development of the Site-Specific in Vivo Analysis System for Mammary Stromal Cells

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  • NAKATANI Hajime
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
  • AOKI Naohito
    Department of Life Sciences, Graduate School of Bioresources, Mie University
  • NADANO Daita
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
  • MATSUDA Tsukasa
    Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University

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  • Transplantation of a Mammary Stromal Cell Line into a Mammary Fat Pad: Development of the Site-Specific<i>in Vivo</i>Analysis System for Mammary Stromal Cells

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Abstract

The interaction between mammary epithelial and stromal tissue is considered to be important in breast tissue development. In this study, we developed a transplantation procedure for the mammary stromal fibroblastic cell line (MSF) to examine its life in vivo. First we established MSF cells which stably expressed lacZ (lacZ/MSF) and had characteristics of mammary stromal cells. The lacZ/MSF cells were then transplanted into a cleared mammary fat pad of syngenic mice with and without mammary primary epithelial organoids. Whole mount X-gal and carmine staining of the transplants revealed that a number of undifferentiated lacZ/MSF cells survived around the mammary epithelial tissue when transplanted with organoids. These results indicate that transplantation of MSF cells into mammary fat pad was accomplished by co-transplantation with primary mammary organoids. Finally, we discuss the application of transplantation procedure for in vivo studies of the mammary stromal tissue development and stromal-epithelial interactions.

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