Immobilization of a Thermostable Cellobiose 2-Epimerase from Rhodothermus marinus JCM9785 and Continuous Production of Epilactose

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  • Immobilization of a Thermostable Cellobiose 2-Epimerase from <i>Rhodothermus marinus</i> JCM9785 and Continuous Production of Epilactose

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Cellobiose 2-epimerase (CE) efficiently forms epilactose which has several beneficial biological functions. A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column. Lactose (100 g/L) was supplied to the reactor, kept at 50 °C at a space velocity of 8 h−1. The epilactose concentration of the resulting eluate was 30 g/L, and this was maintained for 13 d.

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