Macrophage mediated anti-proliferation effects of Anthodia camphorata non-polysaccharide based extracts on human hepatoma cells

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  • CHANG Chia-Yu
    Institute of Biotechnology, College of Engineering, Southern Taiwan University Department of Food Science and Biotechnology, National Chung Hsing University Department of Neurology, Chi Mei Medical Center
  • CHENG Tain-Junn
    Department of Neurology, Chi Mei Medical Center Department of Occupational Medicine, Chi Mei Medical Center Department of Occupational Safety, College of Environment, Chia Nan University of Pharmacy and Science Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University
  • CHANG Fang-Rong
    Graduate Institute of Natural Products, School of Pharmacy, Kaohsiung Medical University
  • WANG Hsien-Yi
    Department of Nephrology, Chi-Mei Medical Center Department of Sports Management, College of Leisure and Recreation Management, Chia Nan University of Pharmacy and Science
  • KAN Wei-Chih
    Department of Nephrology, Chi-Mei Medical Center Department of Medical Laboratory Science and Biotechnology, Chung Hwa University of Medical Technology
  • LI Shun-Lai
    Institute of Biotechnology, College of Engineering, Southern Taiwan University
  • HUANG Li-Hsueh
    Institute of Biotechnology, College of Engineering, Southern Taiwan University Department of Nephrology, Sin-Lau Christian Hospital
  • CHEN Yu-Chun
    Institute of Biotechnology, College of Engineering, Southern Taiwan University
  • TSAI Wan-Chen
    Institute of Biotechnology, College of Engineering, Southern Taiwan University
  • HUANG Chia-Hsin
    Institute of Biomedical Engineering, College of Engineering, National Cheng Kung University Biotechnology Division, Agricultural Research Institute
  • CHENG Chia-Hui
    Institute of Biotechnology, College of Engineering, Southern Taiwan University
  • LEE Guo-Yang
    Institute of Biotechnology, College of Engineering, Southern Taiwan University
  • SHYUE Shiang-Wei
    Institute of Biotechnology, College of Engineering, Southern Taiwan University
  • CHEN Yi-Peng
    Institute of Biotechnology, College of Engineering, Southern Taiwan University
  • LIN Kao-Chang
    Institute of Biotechnology, College of Engineering, Southern Taiwan University Department of Neurology, Chi Mei Medical Center
  • CHUU Jiunn-Jye
    Institute of Biotechnology, College of Engineering, Southern Taiwan University Pharmacy, Wei Gong Memorial Hospital

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  • Macrophage Mediated Anti-Proliferation Effects of <I>Anthodia camphorata</I> Non-Polysaccharide Based Extracts on Human Hepatoma Cells

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It has been reported that medicinal mushrooms might induce different types of immune responses. Anthodia camphorata (A. camphorata) has attracted much attention for its therapeutic effects in treating hepatoma. We tested this anti-tumor effects using immunomodulation of macrophages and extracts of A. camphorata. We evaluated the anti-proliferation effects of various extracts of A. camphorata from fruiting bodies (AC-FB), mycelium of solid-state cultures (AC-SS), liquid-state cultures (AC-LS) and polyaccharide extracts from liquid-state cultures (AC-PS), and extracts of A. camphorata stimulated RAW 264.7 macrophage cell-conditioned mediums (MC-CMs). We measured cell proliferation and, did migration assays by cell cycle analysis and by observing apoptosis-related proteins (AKT, PARP-1, and NF-κB) and the mRNA expression of cytokines (TNF-α and IL-1β) of macrophages in human hepatoma cell lines. Our results revealed that two of the extracts (AC-FB and AC-SS) had better anti-proliferation effects, implying an immunomodulatory role the macrophages might play. This outcome is consistent with findings that AC-FB and AC-SS increase mRNA expression of TNF-α and the corresponding expression of apoptosis-related proteins on activation of MC-CMs, while A. camphorata polysaccharides induce macrophage-derived anti-tumor activities in human hepatoma cells via IL-1β and Akt activation. These results indicate that anti-tumor effects exerted by modulation of macrophage activation of A. camphorate may be influenced by the other constituents which (contained little or no polysaccharide) of A. camphorata.

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