Utilization of Lactic Acid Bacterial Genes in Synechocystis sp. PCC 6803 in the Production of Lactic Acid

DOI Web Site Web Site PubMed 被引用文献3件 参考文献35件
  • JOSEPH Ancy
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
  • AIKAWA Shimpei
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University Core Research for Evolutional Science and Technology, Japan Science and Technology Agency
  • SASAKI Kengo
    Organization of Advanced Science and Technology, Kobe University
  • TSUGE Yota
    Organization of Advanced Science and Technology, Kobe University
  • MATSUDA Fumio
    Organization of Advanced Science and Technology, Kobe University RIKEN Biomass Engineering Program
  • TANAKA Tsutomu
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University
  • KONDO Akihiko
    Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University Core Research for Evolutional Science and Technology, Japan Science and Technology Agency RIKEN Biomass Engineering Program

書誌事項

タイトル別名
  • Utilization of Lactic Acid Bacterial Genes in <i>Synechocystis</i> sp. PCC 6803 in the Production of Lactic Acid
  • Utilization of lactic acid bacterial genes in <italic>Synechocystis</italic> sp. PCC 6803 in the production of lactic acid

この論文をさがす

抄録

Metabolic pathway engineering of cyanobacteria for the production of industrially important chemicals from atmospheric CO2 has generated interest recently. Here, we engineered Synechocystis sp. PCC 6803 to produce lactic acid using a lactate dehydrogenase (ldh) gene from various lactic acid-producing bacteria, Lactococcus lactis (ldhB and ldhX), Lactobacillus plantarum (ldhL and ldh), and Lactobacillus rhamnosus (ldhL). The lactic acid was secreted outside the cell using a transporter (lldp) gene from L. plantarum. Expression of each ldh in Synechocystis sp. PCC6803 was ascertained by reverse-transcriptase polymerase chain reaction. Five transformants led to the production of L-lactic acid. Co-expression of lldp with ldhB from L. plantarum or ldhL from L. rhamnosus led to the secretion of lactic acid into the medium at concentration of 0.17 ± 0.02 or 0.14 ± 0.02 mM after 18 d of cultivation.

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (35)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ