Optimization of Enzymatic Treatment for Compound K Production from White Ginseng Extract by Response Surface Methodology

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  • KIM Eun Hye
    Department of Agricultural Biotechnology, Seoul National University
  • LIM Seokwon
    Department of Food and Biotechnology, Hoseo University
  • KIM Sang Oh
    Department of Agricultural Biotechnology, Seoul National University
  • AHN Sung Ho
    Department of Agricultural Biotechnology, Seoul National University
  • CHOI Young Jin
    Department of Agricultural Biotechnology, Seoul National University Center for Food and Bioconvergence, Seoul National University

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Ginsenoside 20-O-β-D glucopyranosyl-20(S)-protopanaxadiol (compound K), a minor ginsenoside, is not found in white raw ginseng, but has better bioavailability than the major ginsenosides in ginseng. Employing commercial enzyme packages for industrial applications, the optimum conditions for enzymatic transformation for the highest content of compound K was explored to enhance the health benefits of ginseng extract. Cytolase PCL 5 was selected from commercial enzyme packages nominated for high β-glucosidase activity. By response surface methodology, the optimal conditions were identified as 78 h of treatment at pH 4.3 at 55.4 °C for 2.068 mg/mL of compound K, showing good agreement with the experimental value.

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