Aspartyl Aminopeptidase, Encoded by an Evolutionarily Conserved Syntenic Gene, Is Colocalized with Its Cluster in Secretory Granules of Pancreatic Islet Cells
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- CAI Wendy W.
- National Human Genome Center, Howard University College of Medicine International Baccalaureate (IB) Program, Richard Montgomery High School
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- WANG Lin
- Huazhong Normal University
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- CHEN Yuanxiu
- National Human Genome Center, Howard University College of Medicine
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Aspartyl aminopeptidase (DAP), encoded by the DNPEP gene, is believed to be a cytosolic protein with high enzymatic activity in the neuroendocrine tissues. Bioinformatic analysis revealed that the genomic segment spanning the DNPEP gene is evolutionarily conserved from Caenorhabditis elegans to humans. In the present study, we sought to determine whether the expression of DAP is associated with its clustered genes when expressed in pancreatic islet cells. Using anti-DAP specific antibody in immunofluorescent stainings, we found that DAP was specifically expressed in islet alpha cells but not in exocrine acinar cells. Moreover, using electron microscopy, we found that DAP was associated with a lysosomal-like structure and secretory granules, suggesting that it plays an important role in post-translational processing and the secretion of hormones in islet cells. The identification and characterization of DNPEP syntenic genes confirm that conserved clustered genes can preferentially be expressed in the same signaling pathway.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (10), 2050-2055, 2010
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206477329536
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- NII論文ID
- 10027560523
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10860537
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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