Purification, Characterization, and Overexpression of Thermophilic Pectate Lyase of<i>Bacillus</i>sp. RN1 Isolated from a Hot Spring in Thailand
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- SUKHUMSIIRCHART Wasana
- Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University
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- KAWANISHI Sakiko
- Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- DEESUKON Warin
- Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University
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- CHANSIRI Kosum
- Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University
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- KAWASAKI Haruhiko
- Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- SAKAMOTO Tatsuji
- Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
書誌事項
- タイトル別名
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- Purification, Characterization, and Overexpression of Thermophilic Pectate Lyase of Bacillus sp. RN1 Isolated from a Hot Spring in Thailand
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説明
A thermophilic pectate lyase, Pel SWU, was isolated from a culture filtrate of Bacillus sp. RN1 isolated from a hot spring in Ranong Province, Thailand. The enzyme was purified to homogeneity using cation-exchange and hydrophobic column chromatographies. The molecular mass of Pel SWU was estimated to be 33 kDa. The specific substrate was demethylated galacturonic acid. The enzyme was stable at pH 4.0–10.0 and at temperatures up to 70 °C in the presence of calcium and polygalacturonic acid (PGA). The optimum pH and temperature were 10.0 and 90 °C. The pel gene encoding Pel SWU was 1,023 bp, which corresponds to 341 amino acids. The properties of the recombinant enzyme was similar to those of Bacillus Pel SWU. Unsaturated di- and trigalacturonic acids were formed mainly as the final products of degradation by Pel SWU, as revealed by high-performance anion-exchange chromatography (HPAEC) and electrospray ionization mass spectrometry (ESI-MS) analyses. This thermophilic pectate lyase should be useful in the degradation of pectin networks at high temperature.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 73 (2), 268-273, 2009
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206477472000
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- NII論文ID
- 10027536973
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10165944
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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