The Promoter Activity of Isovaleryl-CoA Dehydrogenase-Encoding Gene (ivdA) from Aspergillus oryzae Is Strictly Repressed by Glutamic Acid
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- YAMASHITA Nobuo
- Research & Development Department, Hakutsuru Sake Brewing Co., Ltd.
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- SAKAMOTO Kazutoshi
- National Research Institute of Brewing
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- YAMADA Osamu
- National Research Institute of Brewing
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- AKITA Osamu
- Faculty of Human Life Sciences, Department of Food and Health Sciences, Jissen Women’s University
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- NISHIMURA Akira
- Research & Development Department, Hakutsuru Sake Brewing Co., Ltd.
Bibliographic Information
- Other Title
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- The promoter activity of isovaleryl-CoA dehydrogenase-encoding gene (ivdA) from Aspergillus oryzae is strictly replessed by glutamic acid
- The Promoter Activity of Isovaleryl-CoA Dehydrogenase-Encoding Gene (<i>ivdA</i>) from<i>Aspergillus oryzae</i>Is Strictly Repressed by Glutamic Acid
- The promoter activity of isovareryl-coA dehydrogenase-encodeing gene (ivdA) from Aspergillus oryzae is strictly repressed by glutamic acid
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Abstract
We cloned the isovaleryl-CoA dehydrogenase (IVD)-encoding gene from Aspergillus oryzae. The promoter of ivdA was subjected to β-glucuronidase (GUS) reporter assays in which certain amino acids were used as a major carbon source. L-leucine most strongly induced GUS-activity, while in the case of L-glutamate, significantly low activity was found, indicating that ivdA transcription was strongly repressed by glutamic acid.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (6), 1561-1563, 2007
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001206477530368
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- NII Article ID
- 10027516171
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD2sXnslyqurY%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 8796346
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- PubMed
- 17587691
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed