Isolation of Protoplast from Aerial Mycelia and Fruit-Body Formation from Regenerated Mycelia in Pleurotus cornucopiae
-
- MIZOGUCHI Masahide
- Department of Biology, Faculty of Science and Engineering, Konan University
-
- KITANO Satoshi
- Department of Biology, Faculty of Science and Engineering, Konan University
-
- TAKAHASHI Wataru
- Forage Crop Research Institute, Japan Grassland Agriculture and Forage Seed Association
-
- SUGAWARA Fuyuki
- Akita Prefecture Forest Technical Center
-
- TANAKA Osamu
- Department of Biology, Faculty of Science and Engineering, Konan University
Bibliographic Information
- Other Title
-
- Isolation of Protoplast from Aerial Mycelia and Fruit-Body Formation from Regenerated Mycelia in<i>Pleurotus cornucopiae</i>
Search this article
Abstract
We have developed a simple and efficient method for protoplast isolation from Pleurotus cornucopiae. Protoplasts were isolated from aerial mycelia cultured on potato dextrose agar medium without time-consuming propagation in liquid culture. Protoplast yield was significantly increased by means of a decompressing pretreatment of mycelia in enzyme solution and a subsequent enzyme reaction with vibrational mixing. The isolated protoplasts regenerated mycelia and these mycelia formed fruit-bodies without any morphological abnormalities.
Journal
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 70 (11), 2751-2753, 2006
Japan Society for Bioscience, Biotechnology, and Agrochemistry
- Tweet
Details 詳細情報について
-
- CRID
- 1390001206477550592
-
- NII Article ID
- 10018525071
-
- NII Book ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- NDL BIB ID
- 8560748
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed