High-Performance Liquid Chromatography-Based Multivariate Analysis to Predict the Estrogenic Activity of an Epimedium koreanum Extract

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  • ISLAM M. Nurul
    School of Biology and Environmental Science, University College Dublin School of Biology and Environmental Science, University College Dublin
  • KIM Unyong
    Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University
  • KIM Dong-Hyun
    Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine
  • DONG Mi-Sook
    School of Life Sciences and Biotechnology, Korea University School of Life Sciences, Korea University
  • YOO Hye Hyun
    College of Pharmacy, Hanyang University College of Pharmacy, Hanyang University

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  • High-Performance Liquid Chromatography-Based Multivariate Analysis to Predict the Estrogenic Activity of an <i>Epimedium koreanum</i> Extract

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This study characterizes the correlation between the chemical fingerprint and estrogenic activity of an Epimedium koreanum extract. The estrogenic activity of 31 E. koreanum extract samples was evaluated by a luciferase reporter gene assay, and the samples were classified into 3 groups based on their bioactivity. A chemical fingerprint analysis was performed on each sample by high-performance liquid chromatography (HPLC), and 44 common peaks were selected from the chromatogram and used as a dataset for a pattern recognition analysis. A canonical discriminant analysis performed on this dataset determined a distinct distribution of the samples according to their estrogenic activity on the scoring plot. The classification results showed that 90.3% of the original grouped cases had been correctly classified. The total content of the 4 major extract compounds, epimedin A, epimedin B, epimedin C, and icariin, exhibited good correlation (r=0.784) with the estrogenic activities of the respective extracts. This chromatographic fingerprint-chemometric analysis system could be useful for predicting the E. koreanum pharmacological activity and consequent biological activity-relevant quality control assessment.

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