PiggyBac Transposon-Mediated Gene Transfer in Cashmere Goat Fetal Fibroblast Cells
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- BAI Ding-Ping
- College of Animal Science and Technology, Northwest Agriculture and Forest University College of Animal Science and Technology, Northwest Agriculture and Forest University
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- YANG Ming-Ming
- College of Animal Science and Technology, Northwest Agriculture and Forest University College of Animal Science and Technology, Northwest Agriculture and Forest University
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- CHEN Yu-Lin
- College of Animal Science and Technology, Northwest Agriculture and Forest University College of Animal Science and Technology, Northwest Agriculture and Forest University
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PiggyBac (PB) has recently been found to be functional in various organisms. To verify and exploit its application in the cashmere goat, a PB transposon system including donor and helper vector of was developed, in which the EGFP gene in donor of vector was used as reporter. Cashmere goat fetal fibroblasts cells (GFFs) were transfected with the PB transposon system and the efficiency of gene transfer was determined. Compared with random integration, PB-mediated EGFP expression levels increased 7.78-fold in the GFFs, confirming that the PB transposon system constructed successfully mediated efficient foreign gene integration in the GFFs. To further investigate the characteristics of PB-mediated integration instance, PB integration site distribution in the goat genome was examined. The results showed that PB had a preference for AT rich regions of the goat genome. Thus this study confirms the function of PB transposon in GFFs and provides a potential genetic tool for producing transgenic goats.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (5), 933-937, 2012
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206477617920
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- NII論文ID
- 10030752736
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC38jltlOgtQ%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 023681903
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- PubMed
- 22738962
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可