Selection Dynamic of<i>Escherichia coli</i>Host in M13 Combinatorial Peptide Phage Display Libraries

DOI Web Site Web Site 参考文献31件
  • ZANCONATO Stefano
    European Centre for Living Technology, Ca’ Foscari University Venice
  • MINERVINI Giovanni
    European Centre for Living Technology, Ca’ Foscari University Venice
  • POLI Irene
    European Centre for Living Technology, Ca’ Foscari University Venice Department of Statistics, Ca’ Foscari University Venice
  • LUCREZIA Davide De
    European Centre for Living Technology, Ca’ Foscari University Venice

書誌事項

タイトル別名
  • Selection Dynamic of Escherichia coli Host in M13 Combinatorial Peptide Phage Display Libraries

この論文をさがす

抄録

Phage display relies on an iterative cycle of selection and amplification of random combinatorial libraries to enrich the initial population of those peptides that satisfy a priori chosen criteria. The effectiveness of any phage display protocol depends directly on library amino acid sequence diversity and the strength of the selection procedure. In this study we monitored the dynamics of the selective pressure exerted by the host organism on a random peptide library in the absence of any additional selection pressure. The results indicate that sequence censorship exerted by Escherichia coli dramatically reduces library diversity and can significantly impair phage display effectiveness.

収録刊行物

参考文献 (31)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ