Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein of<scp>D</scp>-Amino Acid Oxidase with<i>Vitroscilla</i>Hemoglobin
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- SEO Young-Man
- School of Biotechnology, Yeungnam University
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- KHANG Yong-Ho
- School of Biotechnology, Yeungnam University
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- YUN Hyungdon
- School of Biotechnology, Yeungnam University
書誌事項
- タイトル別名
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- Kinetic Resolution of 3-Fluoroalanine Using a Fusion Protein of D-Amino Acid Oxidase with Vitroscilla Hemoglobin
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In this study, a fusion protein (VHb-DAAO) of D-amino acid oxidase (DAAO) with Vitreoscilla hemoglobin (VHb) was functionally expressed in Escherichia coli and purified. The kcat value VHb-DAAO (47.1 s−1) towards rac-3-flouroalanine was about 2-fold higher than that of DAAO (21.9 s−1). rac-3-Flouroalanine (500 mM) was kinetically resolved into (R)-3-fluoroalanine with high enatiomeric excess (>99%) by VHb-DAAO with about 52% conversion.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 75 (4), 820-822, 2011
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206477722496
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- NII論文ID
- 10028272128
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 11071263
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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