Comparison of Components and Synthesis Genes of Cell Wall Teichoic Acid among<i>Lactobacillus plantarum</i>Strains

  • TOMITA Satoru
    Department of Applied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture
  • IRISAWA Tomohiro
    Department of Applied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture
  • TANAKA Naoto
    Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture
  • NUKADA Tomoo
    Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture
  • SATOH Eiichi
    Department of Applied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture
  • UCHIMURA Tai
    Department of Applied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture
  • OKADA Sanae
    Department of Applied Biology and Chemistry, Faculty of Applied Bio-Science, Tokyo University of Agriculture

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  • Comparison of Components and Synthesis Genes of Cell Wall Teichoic Acid among Lactobacillus plantarum Strains

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The contents, components, and synthesis genes of cell wall teichoic acid (WTA) in 18 strains of Lactobacillus plantarum were compared. The WTA of each strain was classified by its components as being either the glycerol- or the ribitol-type. The different strains in the WTA type showed marked differences also in two gene regions, tagD1–tagF2 and lp_1816–tagB2, as for the presence or absence, nucleotide sequences, and transcriptional activities. Our results clearly showed that the tagD1–tagF2 and lp_1816–tagB2 regions contained the synthesis genes of the WTA backbone of L. plantarum. We verified that the genes in the tagD1–tagF2 region were involved in the synthesis of the glycerol-type backbone. Furthermore, we propose that the genes in the lp_1816–tagB2 region were tarI, tarJ, tarK, and tarL, which are involved in the synthesis of the ribitol-type backbone.

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