Mammalian ESCRT-Ⅲ-Related Protein IST1 Has a Distinctive Met-Pro Repeat Sequence That Is Essential for Interaction with ALG-2 in the Presence of Ca²⁺
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- OKUMURA Mayumi
- Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
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- TAKAHASHI Takeshi
- Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
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- SHIBATA Hideki
- Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
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- MAKI Masatoshi
- Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
書誌事項
- タイトル別名
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- Mammalian ESCRT-III-Related Protein IST1 Has a Distinctive Met-Pro Repeat Sequence That Is Essential for Interaction with ALG-2 in the Presence of Ca<sup>2+</sup>
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説明
ALG-2 is an EF-hand-type Ca2+-binding protein that interacts with a variety of intracellular proteins that possess Pro-rich regions (PRRs) in mammalian cells. IST1 is an endosomal sorting complex required for transport (ESCRT)-III-related charged multivesicular body protein (CHMP)-like protein, but unlike other ESCRT-III proteins, mammalian IST1 has a PRR and a distinctive sequence of Met-Pro repeats. We found that ALG-2 binds to IST1 by Far-Western analysis using biotinylated ALG-2 as probe, and that the Met-Pro repeat sequence is essential for interaction. The results of pulldown assays using Strep-tagged ALG-2 and lysates of cells expressing GFP-fused IST1 proteins indicated that the binding of ALG-2 to IST1 is Ca2+-dependent, and that it is enhanced by co-expression with CHMP1 proteins. Moreover, pulldown assays using various mutants of GST-ALG-2 revealed that the ability of IST1 to bind to mutants is different from those of known ALG-2-interacting proteins, suggesting that IST1 binds to ALG-2 by a different mode of recognition.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (5), 1049-1054, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206477974016
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- NII論文ID
- 10031177595
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3snht1Chsg%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024526190
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- PubMed
- 23649269
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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