Purification and Characterization of Membrane-Bound 3-Dehydroshikimate Dehydratase from Gluconobacter oxydans IFO 3244, A New Enzyme Catalyzing Extracellular Protocatechuate Formation

DOI Web Site Web Site 4 Citations 23 References
  • SHINAGAWA Emiko
    Department of Chemical and Biological Engineering, Ube National College of Technology
  • ADACHI Osao
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
  • ANO Yoshitaka
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
  • YAKUSHI Toshiharu
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University
  • MATSUSHITA Kazunobu
    Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University

Bibliographic Information

Other Title
  • Purification and Characterization of Membrane-Bound 3-Dehydroshikimate Dehydratase from<i>Gluconobacter oxydans</i>IFO 3244, A New Enzyme Catalyzing Extracellular Protocatechuate Formation

Search this article

Abstract

3-Dehydroshikimate dehydratase (DSD) is the first known enzyme catalyzing aromatization from 3-dehydroshikimate (DSA) to protocatechuate (PCA). Differently from cytosolic DSD (sDSD), a membrane-bound 3-dehydroshikimate dehydratase (mDSD) was found for the first time in the membrane fraction of Gluconobacter oxydans IFO 3244, and DSA was confirmed to be the direct precursor of PCA. In contrast to weak and instable sDSD, the abundance of mDSD in the membrane fraction suggested the metabolic significance of mDSD as the initial step in aromatization. mDSD was solubilized only by a detergent and was readily purified to high homogeneity. Its molecular weight was estimated to be 76,000. Purified mDSD showed a sole peak at 280 nm in the absorption spectrum and no critical cofactor requirements. The Km of DSA was measured at 0.5 mM, and the optimum pH was observed at pH 6–8. mDSD appeared to react only with DSA, and was inert to other compounds, such as 3-dehydroquinate, quinate, and shikimate.

Journal

Citations (4)*help

See more

References(23)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top