Deletion Analysis of the Catalase-Encoding Gene (<i>catB</i>) Promoter from<i>Aspergillus oryzae</i>

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  • HISADA Hiromoto
    Research Institute, Gekkeikan Sake Co., Ltd.
  • SANO Motoaki
    Gene Regulation Group, Molecular and Cell Biology, National Institute of Advanced Industrial Science and Technology (AIST)
  • HATA Yoji
    Research Institute, Gekkeikan Sake Co., Ltd.
  • ABE Yasuhisa
    Research Institute, Gekkeikan Sake Co., Ltd.
  • MACHIDA Masayuki
    Gene Regulation Group, Molecular and Cell Biology, National Institute of Advanced Industrial Science and Technology (AIST)

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  • Deletion Analysis of the Catalase-Encoding Gene (catB) Promoter from Aspergillus oryzae

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説明

The catalase-encoding gene (catB) is expressed strongly in Aspergillus oryzae. To identify the transcription regulatory elements involved in strong expression, we did promoter deletion analysis using β-glucuronidase (GUS) as a reporter and an electrophoretic gel mobility shift assay (EMSA) systematically. The deletion 200-bp sequence from −1,000 to −800 in the 1,400-bp catB promoter caused a drastic decrease in GUS activity. In addition, EMSA implicated a 45-bp element from −1,000 to −956 containing cis-elements. According to detailed promoter deletion analysis, a region from −1,000 to −975, which contains putative heat shock element (HSE) and the CCAAT-box, was involved in strong expression.

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