Purification and Characterization of a 56 kDa Chitinase Isozyme (PaChiB) from the Stomach of the Silver Croaker, Pennahia argentatus
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- IKEDA Mana
- Department of Marine Science and Resources, College of Biosource Sciences, Nihon University Department of Marine Science and Resources, College of Biosource Sciences, Nihon University
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- MIYAUCHI Kouji
- Department of Marine Science and Resources, College of Biosource Sciences, Nihon University Department of Marine Science and Resources, College of Biosource Sciences, Nihon University
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- MATSUMIYA Masahiro
- Department of Marine Science and Resources, College of Biosource Sciences, Nihon University Department of Marine Science and Resources, College of Biosource Sciences, Nihon University
書誌事項
- タイトル別名
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- Purification and Characterization of a 56 kDa Chitinase Isozyme (PaChiB) from the Stomach of the Silver Croaker, <i>Pennahia argentatus</i>
- Purification and characterization of a 56kDa chitinase isozyme (PaChiB) from the stomach of silver croaker Pennahia argentatus
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A 56 kDa chitinase isozyme (PaChiB) was purified from the stomach of the silver croaker Pennahia argentatus. The optimum pH and pH stability of PaChiB were observed in an acidic pH range. When N-acetylchitooligosaccharides ((GlcNAc)n, n=2 –6) were used as substrates, PaChiB degraded (GlcNAc)4 –6 and produced (GlcNAc)2,3. It degraded (GlcNAc)5 to produce (GlcNAc)2 (23.2%) and (GlcNAc)3 (76.8%). The ability to degrade p-nitrophenyl N-acetylchitooligosaccharides (pNp-(GlcNAc)n, n=2 –4) fell in the following order: pNp-(GlcNAc)3 ≫ pNp-(GlcNAc)2 > pNp-(GlcNAc)4. Based on these results, we concluded that PaChiB is an endo-type chitinolytic enzyme, and that it preferentially hydrolyzes the third glycosidic bond from the non-reducing end of (GlcNAc)n. Activity toward crystalline α- and β-chitin was activated at 124%–185% in the presence of 0.5 M NaCl. PaChiB exhibited markedly high substrate specificity toward crab-shell α-chitin.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 76 (5), 971-979, 2012
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206478301952
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- NII論文ID
- 10030752947
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC38jltlOntg%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 023681951
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- PubMed
- 22738969
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可