Electron Microscopic Analysis of Heat-Induced Leakage of Polyphosphate from a phoU Mutant of Escherichia coli

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  • KASHIHARA Hitoshi
    Department of Biotechnology, Graduate School of Engineering, Osaka University
  • KANG Bong Mun
    Department of Biotechnology, Graduate School of Engineering, Osaka University
  • OMASA Takeshi
    Department of Biotechnology, Graduate School of Engineering, Osaka University
  • HONDA Kohsuke
    Department of Biotechnology, Graduate School of Engineering, Osaka University
  • SAMESHIMA Yuka
    Department of Biotechnology, Graduate School of Engineering, Osaka University
  • KURODA Akio
    Department of Molecular Biotechnology, Hiroshima University
  • OHTAKE Hisao
    Department of Biotechnology, Graduate School of Engineering, Osaka University

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  • Electron Microscopic Analysis of Heat-Induced Leakage of Polyphosphate from a<i>phoU</i>Mutant of<i>Escherichia coli</i>

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Abstract

Heat treatment is a simple technique for releasing polyphosphate (polyP) from microbial cells. We investigated the ultrastructural alterations of polyP granules (PPGs) in response to heat treatment at 70 °C using a polyP-accumulating phoU mutant of Escherichia coli as a model organism. Electron microscopic and energy-dispersive X-ray analysis suggested concurrent occurrence of PPG degradation and polyP release in the cytoplasm. PolyP is probably released by cytoplasmic extrusion through breaks in the cell envelope.

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