Identification of a new pheromone-binding protein in the antennae of a geometrid species and preparation of its antibody to analyze the antennal proteins of moths secreting type 2 sex pheromone components

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  • WATANABE Hayaki
    Graduate School of Bio-Applications and Systems Engineering (BASE), Tokyo University of Agriculture and Technology
  • TABUNOKI Hiroko
    Graduate School of Bio-Applications and Systems Engineering (BASE), Tokyo University of Agriculture and Technology
  • MIURA Nami
    Graduate School of Bio-Applications and Systems Engineering (BASE), Tokyo University of Agriculture and Technology
  • MATSUI Aya
    Graduate School of Bio-Applications and Systems Engineering (BASE), Tokyo University of Agriculture and Technology
  • SATO Ryoichi
    Graduate School of Bio-Applications and Systems Engineering (BASE), Tokyo University of Agriculture and Technology
  • ANDO Tetsu
    Graduate School of Bio-Applications and Systems Engineering (BASE), Tokyo University of Agriculture and Technology

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タイトル別名
  • Identification of a New Pheromone-Binding Protein in the Antennae of a Geometrid Species and Preparation of Its Antibody to Analyze the Antennal Proteins of Moths Secreting Type II Sex Pheromone Components

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説明

The full-length cDNA sequence of a new pheromone-binding protein (AscrPBP2) was determined from a geometrid moth, Ascotis selenaria cretacea, which secreted a Type II sex pheromone, and an antiserum against its recombinant protein overexpressed in Escherichia coli was prepared. In addition to this antiserum against AscrPBP2, antibodies against AscrPBP1 and general odorant-binding proteins of Bombyx mori were used in Western blotting experiments to analyze the proteins in the antennae of several lepidopteran species secreting Type II sex pheromone components.

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