A Eukaryotic Molecular Target Candidate of Roxithromycin: Fungal Differentiation as a Sensitive Drug Target Analysis System
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- ISHII Akira
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- KUMASAKA Mayu
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- NAGASHIMA Yuka
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- NAKAJIMA Yuichi
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- KURAMOCHI Kouji
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- SUGAWARA Fumio
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- NARUKAWA Megumi
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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- KAMAKURA Takashi
- Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science
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抄録
Roxithromycin (RXM), active against prokaryotes, has beneficial side effects such as anti-cancer activities on mammalian cells, but the mechanisms underlying these effects remain unclear. We found that RXM inhibited the cellular differentiation of the rice blast fungus Magnaporthe oryzae. Hence, we screened the targets of RXM by the T7 phage display method with fungal genomic DNA, and identified MoCDC27 (M. oryzae Cell Division Cycle 27) as a candidate. We generated mocdc27 knockdown mutants that the appressoria formation was less affected by RXM. A complemented mutant restored sensitivity against RXM to the level of the wild type. These results suggest that MoCDC27 was involved in the inhibition of appressorium formation by RXM, and that the complex of RXM-MoCDC27 affected another molecule involved in appressorium formation. The T7 phage display method with fungal genomic DNA can be a useful tool in the quest for drug target.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 77 (7), 1539-1547, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479006592
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- NII論文ID
- 10031190466
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3sjos1eisA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024723754
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- PubMed
- 23832352
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可