Functional Analysis of A Pyoverdine Synthetase from<i>Pseudomonas</i>sp. MIS38
-
- LIM Siew Ping
- Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University
-
- ROONGSAWANG Niran
- Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University
-
- WASHIO Kenji
- Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University
-
- MORIKAWA Masaaki
- Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University
書誌事項
- タイトル別名
-
- Functional Analysis of A Pyoverdine Synthetase from Pseudomonas sp. MIS38
この論文をさがす
抄録
Fluorescent Pseudomonas sp. MIS38 produces a cyclic lipopeptide, arthrofactin. Arthrofactin is synthesized by a unique nonribosomal peptide synthetase (NRPS) with dual C/E-domains. In this study, another class of cyclic peptide, pyoverdine, was isolated from MIS38, viz., Pvd38. The main fraction of Pvd38 had an m⁄z value of 1,064.57 and contained Ala, Glu, Gly, (OHOrn), Ser, and Thr at a ratio of 2:1:1:(1):1:1 in the peptide part, suggesting a new structure compound. A gene encoding NRPS for the chromophore part of Pvd38 was identified, and we found that it contained a conventional E-domain. Gene disruption completely impaired the production of Pvd38, demonstrating that the synthetase is functional. This observation allows us to conclude that different NRPS systems with dual C/E-domains (in arthrofactin synthetase) and a conventional E-domain (in pyoverdine synthetase) are both functional in MIS38.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 71 (8), 2002-2009, 2007
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001206479019520
-
- NII論文ID
- 10027517861
-
- NII書誌ID
- AA10824164
-
- ISSN
- 13476947
- 09168451
-
- HANDLE
- 2115/29888
-
- NDL書誌ID
- 8887750
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可