Identification of the Functional Periplasmic Nitrate Reductase (<i>nap</i>) Gene Cluster from the Deep-Sea Denitrifier<i>Pseudomonas</i>sp. Strain MT-1
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- TAMEGAI Hideyuki
- Department of Chemistry, College of Humanities and Sciences, Nihon University
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- IKEDA Eriko
- Department of Chemistry, College of Humanities and Sciences, Nihon University
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- KATO Chiaki
- Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
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- HORIKOSHI Koki
- Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
書誌事項
- タイトル別名
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- Identification of the Functional Periplasmic Nitrate Reductase (nap) Gene Cluster from the Deep-Sea Denitrifier Pseudomonas sp. Strain MT-1
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The nap gene cluster encoding periplasmic nitrate reductase was identified from Pseudomonas sp. strain MT-1, a deep-sea denitrifier isolated from the Mariana Trench. The ORFs identified were highly homologous with those of Pseudomonas stutzeri, but the cluster included only four ORFs (napDABC), less than those in other organisms. For other bacteria, some additional small ORFs (such as napE, napF, napG, napH, and napK) are found in the nap gene cluster, although their physiological function is still unclear. The soluble fraction of MT-1 grown under denitrifying condition showed significant nitrate reductase activity. This observation suggests that the periplasmic nitrate reductase encoded by the gene cluster identified in this study is functional. The activity was highest when the organism was grown under denitrifying conditions, suggesting that the enzyme participates in dissimilatory nitrite reduction.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (8), 2041-2045, 2007
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479025920
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- NII論文ID
- 10027517994
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8888034
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 使用不可