Increased Lysine Content in Rice Grains by Over-Accumulation of BiP in the Endosperm

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  • KAWAKATSU Taiji
    Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences
  • WANG Shuyi
    Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences
  • WAKASA Yuhya
    Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences
  • TAKAIWA Fumio
    Transgenic Crop Research and Development Center, National Institute of Agrobiological Sciences

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Over-accumulation of lysine-rich binding protein (BiP) in the rice endosperm caused strong endoplasmic reticulum (ER) stress and reduced seed storage proteins, resulting in a relative increase in nutritionally balanced non-seed storage proteins. We show that transgenic rice with over-accumulated BiP was a high-lysine rice germplasm and that the over-accumulation of BiP in the endosperm offered a unique strategy to improve the lysine content of cereal grains.

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