Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs in Transgenic Analysis of Plants
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- NAKAGAWA Tsuyoshi
- Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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- SUZUKI Takamasa
- Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
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- MURATA Satoko
- Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
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- NAKAMURA Shinya
- Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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- HINO Takeshi
- Department of Molecular and Functional Genomics, Center for Integrated Research in Science, Shimane University
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- MAEO Kenichiro
- Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
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- TABATA Ryo
- Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
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- KAWAI Tsutae
- Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
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- TANAKA Katsunori
- Department of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
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- NIWA Yasuo
- Laboratory of Plant Cell Technology, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
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- WATANABE Yuichiro
- Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
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- NAKAMURA Kenzo
- Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
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- KIMURA Tetsuya
- Department of Sustainable Resource Science, Faculty of Bioresources, Mie University
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- ISHIGURO Sumie
- Department of Biological Functions and Mechanisms, Graduate School of Bioagricultural Sciences, Nagoya University
Bibliographic Information
- Other Title
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- Improved gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plans
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Abstract
We made a series of improved Gateway binary vectors (pGWBs) for plant transformation. Fifteen different reporters and tags, sGFP, GUS, LUC, EYFP, ECFP, G3GFP, mRFP, 6×His, FLAG, 3×HA, 4×Myc, 10×Myc, GST, T7, and TAP, were employed. Some vectors carry the 2×35S-Ω promoter for higher-level expression. The kanamycin- and hygromycin-resistant markers are independently available for each of the 43 types of vectors, thus an additional transformation of once-transformed plants can be carried out easily. Their small size and high-copy number in Escherichia coli make possible easier handling at plasmid preparation and sequencing. Improved pGWBs should be a powerful tool for transgenic research in plants.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 71 (8), 2095-2100, 2007
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206479098880
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- NII Article ID
- 10027518220
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD2sXhtVaisr%2FL
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 8888342
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- PubMed
- 17690442
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed
