Enhanced Valine Production in<i>Corynebacterium glutamicum</i>with Defective H<sup>+</sup>-ATPase and C-Terminal Truncated Acetohydroxyacid Synthase
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- WADA Masaru
- Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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- HIJIKATA Nowaki
- Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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- AOKI Ryo
- Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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- TAKESUE Nobuchika
- Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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- YOKOTA Atsushi
- Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
書誌事項
- タイトル別名
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- Enhanced Valine Production in Corynebacterium glutamicum with Defective H+-ATPase and C-Terminal Truncated Acetohydroxyacid Synthase
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説明
We have reported increased glutamate production by a mutant of Corynebacterium glutamicum ATCC14067 (strain F172-8) with reduced H+-ATPase activity under biotin-limiting culture conditions (Aoki et al. Biosci. Biotechnol. Biochem., 69, 1466–1472 (2005)). In the present study, we examined valine production by an H+-ATPase-defective mutant of C. glutamicum. Using the double-crossover chromosome replacement technique, we constructed a newly defined H+-ATPase-defective mutant from ATCC13032. After transforming the new strain (A-1) with a C-terminal truncation of acetohydroxyacid synthase gene (ilvBN), valine production increased from 21.7 mM for the wild-type strain to 46.7 mM for the A-1 in shaking flask cultures with 555 mM glucose. Increased production of the valine intermediate acetoin was also observed in A-1, and was reduced by inserting acetohydroxyacid isomeroreductase gene (ilvC) into the ilvBN plasmid. After transformation with this new construct, valine production increased from 38.3 mM for the wild-type strain to 95.7 mM for A-1 strain. To the best of our knowledge, this is the first report indicating that an H+-ATPase-defective mutant of C. glutamicum is capable of valine production. Our combined results with glutamate and valine suggest that the H+-ATPase defect is also effective in the fermentative production of other practical compounds.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 72 (11), 2959-2965, 2008
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479234048
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- NII論文ID
- 10027534140
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 9723970
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- PubMed
- 18997402
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- 本文言語コード
- en
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