Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts
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- SAKAMOTO Shingo
- Graduate School of Biosphere Sciences, Hiroshima University
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- FUJIKAWA Yukichi
- Graduate School of Biosphere Sciences, Hiroshima University
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- ESAKA Muneharu
- Graduate School of Biosphere Sciences, Hiroshima University
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We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts was increased by transient expression of the L-galactose-1-phosphate phosphatase gene. This system provides a means of rapid analysis to clarify the function of AsA biosynthetic enzymes and AsA roles in tomato fruit.
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 77 (3), 673-675, 2013
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479317504
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- NII論文ID
- 10031164797
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- NII書誌ID
- AA10824164
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- COI
- 1:STN:280:DC%2BC3svkt1CitA%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 024391483
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- PubMed
- 23470746
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
