Expression and Immunological Studies of Classical Swine Fever Virus Glycoprotein E2 in the Bi-Cistronic Baculovirus/Larvae Expression System
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- WU Chi-Ming
- Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
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- HSUAN Shih-Ling
- Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
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- CHEN Zeng-Weng
- Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University
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- JINN Tzyy-Rong
- Graduate Institute of Chinese Medical Science, College of Chinese Medicine, China Medical University
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- HUANG Chienjin
- Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University
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- LIAO Jiunn-Wang
- Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
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- CHEN Ter-Hsin
- Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University
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- LIAO Chih-Ming
- Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
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- LEE Wei-Cheng
- Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
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- WU Tzong-Yuan
- Department of Bioscience Technology, Chung Yuan Christian University
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- CHIEN Maw-Sheng
- Graduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
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抄録
To develop an economical, easy technique for producing recombinant E2 glycoprotein (rE2) of classical swine fever virus (CSFV) as a candidate immunogen, a bi-cistronic baculovirus/larvae expression vector was constructed using p10 promoter, an internal ribosome entry site, and the gfp gene. Trichoplusia ni larvae were successfully infected with the occluded recombinant baculovirus via feed, and the characteristics of rE2 were confirmed by immunoblot and glycosylation stain. rE2 at a concentration of 0.6–0.8 mg/ml without degradation was obtained from hemolymphs of infected larvae that emitted high levels of green fluorescence. Immunization assays indicated that mice and piglets immunized with rE2-containing hemolymph elicited high titers of anti-CSFV E2 antibodies with virus-neutralizing activity. This is the first study to indicate that baculovirus/T. ni larvae-expressed rE2 can be served as a vaccine candidate. This system provides an economical alternative for the production of vaccine components in the veterinary industry.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 74 (7), 1343-1349, 2010
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479381376
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- NII論文ID
- 10027556892
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 10766371
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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